Författare/Namn
Titel Evaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer
Anmärkning: Innehållsbeskrivning, sammanfattning BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series.
Ämne
Medarbetare Dictor, Michael Författare/medförfattare Bendahl, Pär-Ola Författare/medförfattare Fernö, Mårten Författare/medförfattare Nilbert, Mef Författare/medförfattare
Institutionsnamn
Värdpublikation Archives of pathology & laboratory medicine College of American Pathologists ISSN 0003-9985 126:6, s. 702-5 126:6<702-5
Antal i kö:
*00004102naa a22005773a 4500
*00144747
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*008080901s2002 xx |||| |0|| 0|eng d
*0247 $a000176056400011$2isi
*035 $a(SwePub)oai:lup.lub.lu.se:108564
*040 $a(SwePub)lu
*042 $9SwePub
*072 7$aart$2swepub-publicationtype
*072 7$aref$2swepub-contenttype
*1001 $aFernebro, Eva$4aut$0(SwePub:)
*24510$aEvaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer
*5203 $8eng$aBACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series.
*653 $aProtein p53 : analysis
*653 $aRectal Neoplasms : chemistry
*653 $aMiddle Age
*653 $aMale
*653 $aKi-67 Antigen : analysis
*653 $aImmunoenzyme Techniques
*653 $aHuman
*653 $aHistocytological Preparation Techniques : methods
*653 $aFemale
*653 $aCell Count
*653 $aCarcinoma : pathology
*653 $aCarcinoma : chemistry
*653 $aNeedle
*653 $aBiopsy
*653 $aAdult
*653 $aAged
*653 $aRectal Neoplasms : pathology
*653 $aSupport
*653 $aNon-U.S. Gov't
*653 $aTumor Markers
*653 $aBiological : analysis
*653 $aMedicin
*653 $aMedicine
*7001 $aDictor, Michael$4aut$0(SwePub:)
*7001 $aBendahl, Pär-Ola$4aut$0(SwePub:)
*7001 $aFernö, Mårten$4aut$0(SwePub:)
*7001 $aNilbert, Mef$4aut$0(SwePub:)
*7102 $8swe$aLunds universitet.$bMedicin.$bInstitutionen för kliniska vetenskaper, Lund.$bSektion V.$bPatologi, Lund.$0(SwePub:lu)
*7102 $8eng$aLund University.$bFaculty of Medicine .$bDepartment of Clinical Sciences, Lund.$bDivision V.$bPathology, (Lund).$0(SwePub:lu)
*7102 $8swe$aLunds universitet.$bMedicin.$bInstitutionen för kliniska vetenskaper, Lund.$bSektion V.$bOnkologi, Lund.$0(SwePub:lu)
*7102 $8eng$aLund University.$bFaculty of Medicine .$bDepartment of Clinical Sciences, Lund.$bDivision V.$bOncology, Lund.$0(SwePub:lu)
*7730 $tArchives of pathology & laboratory medicine $dCollege of American Pathologists$x0003-9985$g126:6, s. 702-5$q126:6<702-5
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